Maria Lucia Ishida
April 2002 - PhD in Biological Sciences – Biochemistry. Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil.
March
1996 - MSc in Biological Sciences – Biochemistry.
Federal University of Paraná
(UFPR), Curitiba, Paraná,
Brazil.
March
1990 - BS Biology. Federal
University of Paraná
(UFPR), Curitiba, Paraná,
Brazil.
Areas
of Activity
· Food safety (2007)
· Environmental remediation and ecophysiology of rare plants (2004 to 2007)
·
Xylem
fluid chemistry mediation of resistance to Pierce’s disease. (2002 - 2004).
·
A
role of adhesion proteins and exoenzymes in the pathogenicity of the plant
pathogenic bacterium, Xylella fastidiosa. (1999 - 2001).
·
Identification
and characterization of genes products that involved in the nitrogen
metabolism regulatory system of Azospirillum brasilense.
(1993 - 2002).
·
Cytogenesis
analysis of rodent from "Segredo" Reservoir. (1991 - 1992).
·
Effects
of organophosphate pesticides in fishes. (1988 - 1990).
·
Alteration
in biological rhythm of Antarctic’s fishes in different light and
salinity conditions. (1988 - 1990).
·
Effects
of acid water in respiratory behavior of Trichogaster trichopterus.
(1988 - 1990).
Working
Experience
Biological
Scientist.
University of Florida – FL.06/2004
to 06/2007.
North
Florida Research and Education Center, Quincy, FL, US.
Responsible
for design and execute experiments in enviromental remediation and
ecophysiology of rare plants.
Pos-Doctoral Research.
University of Florida – FL.05/2003 to
05/2004.
North
Florida Research and Education Center, Quincy, FL, US.
Responsible
for (1) in vitro culture of Xylella fastidiosa, (2) identification
of X. fastidiosa by molecular biology techniques, (3) isolation and
characterization of X. fastidiosa outer-membrane proteins, and (4)
isolation and characterization of protein from grape xylem fluids.
Biological Scientist.
University of Florida – FL. 09/2002 to 05/2003.
North
Florida Research and Education Center, Quincy, FL, US.
Responsible
for (1) in vitro culture of Xylella fastidiosa, (2) identification
of X. fastidiosa by molecular biology techniques, (3) isolation and
characterization of X. fastidiosa outer-membrane proteins, and (4)
isolation and characterization of protein from grape xylem fluids.
Research
technician. University of São Paulo - USP. 1999 -2001.
Toxicologist. Federal University Parana – UFPR. 1991-1993.
Scholarships
1989/1990 – Scientific Initiation -
RHAE – Biotechnology - MCT(1)/CNPq
066/88
1991/1992 – Scientific Improvement
– CNPq (2) - AP-B 820399/90-6
1993/1995 – Master Scholarship -
CAPES (3)
1996-2000 – Ph.D. Scholarship –
CNPq
(1) MCT =
Ministry of Science and Technology - Brazil
(2) CNPq =
National Council of Science – Brazil
(3)CAPES =
National Coordination for Knowledge Improvement – Brazil
Areas
of Activity
1 – A role of adhesion proteins and exoenzymes in the
pathogenicity of plant bacterium, Xylella fastidiosa.
2 – Identification and characterization of genes products that
participate in the nitrogen metabolism regulatory system of Azospirillum
brasilense.
Awards
/ Distinctions
1
– 5th Science and Technology
Award. Award granted to Maria Lucia Ishida
by the Paraná State Government. Student category. 1991.
2 - Biological Sciences Honor
Distinction, 1991. Granted by the Federal University of Paraná as recognition for
projects concerned with impact of pollutants toxicity on fish.
Professional
Services for Scholarly Journals
Reviewer
for Academic Journals
Revista Brasileira de Botanica (Brazilian Journal of Botany)
Peer-reviewer for in-house manuscripts
University of Florida-NFREC/IFAS, Quincy, Florida
Universidade de Sao Paulo - ESALQ, Piracicaba, SP,
Brazil
Professional
Associations
American
Society of Microbiology - USA
Brazilian Society of Biochemistry and Molecular Biology – Brazil
Seminars and oral presentations
XXVIII Annual Meeting at the Brazilian Society of Biochemistry and
Molecular Biology. Identification and characterization of a new
two-component regulatory system NtrY/NtrX in Azospirillum
brasilense. Caxambú, MG, Brazil. 1999.
Publications
Ishida,
M. L.,
Andersen, P. C. & Leite, B (2004). Cecropin B antimicrobial activity
against Xylella fastidiosa: effect on colony formation.
Physiological and Molecular Plant Pathology. In press.
Leite,
B., Andersen, P. C. & Ishida, M. L. (2004). Colony
aggregation and biofilm formation in xylem chemistry-based media for Xylella
fastidiosa. FEMS Microbial. Letts. 230:283-290.
Andersen,
P.C., Ishida, M.L., Momol, E. A., Brodbeck, B. V., Leite,
B., Momol, M. T. (2004). Influence of Vitis xylem fluid and xylem
fluid plus cecropin B on growth of Xylella fastidiosa. Vitis.
43(1): 19-26.
Ishida, M.L.;
Assumpção, M.C.; Machado, H.B; Benelli, E. M.; Souza, E.M. & Pedrosa,
F. O. (2002). Identification
and characterization of the two-component regulatory system NtrY/NtrX in Azospirillum
brasilense. Braz.
J. Med. Biol. Res., 35(6): 651-61.
Leite,
B.; Ishida, M.L.; Alves, E.; Carrer, H.; Pascholati, S.F. &
Kitajima, E.W. (2002). Genomics and X-ray microanalysis indicate that Ca++
and thiols mediate the Xylella
fastidiosa aggregation and adhesion. Braz.
J. Med. Biol. Res., 35(6): 645-50.
Leite, B. Pascholati, S.F., Kitajima, E.W. & Ishida, M.L. 2001. Mecanismos de adesão de bactérias e fungos às plantas hospedeiras. Revisao Anual de Patologia de Plantas. 9: 119-157. (Portuguese)
Leite, B.
Ishida, M.L., Alves, E.,
Pascholati, S.F. & Sugui, J.A. (2000) Detection of calcium in the
adhesive material obtained from the plant pathogen Colletotrichum
graminicola: X ray microanalysis (EDS) evidences. Proceedings of
Microscopy and Microanalysis. 6: 698-9.
Ishida, M.L.; Machado, H.B.; Souza, E.M.; Yates, M.G. & Pedrosa, F.O. (1999) The Azospirillum
brasilense two-component regulatory system composed by NtrY and NtrX
proteins is involved in the regulation of nitrogen fixation. IN Pedrosa,
F. O.; Yates, M. G., Hungria M. & W.E. Newton eds. Biological Nitrogen
Fixation for the 21st Century, Kluwer Academic Publishers, 1999, p.104. In
12th International Congress on Nitrogen Fixation, Foz do Iguaçu, Paraná,
Brazil.
Pedrosa,
F.O.; Teixeira, K.R.S.; Machado, I.M.P.; Steffens, M.B.R.; Glassen, G.;
Benelli, E.M.; Machado, H.B.; Funayama, S.; Rigo, L.U.; Ishida, M.L.;
Yates, M.G. & Souza, E.M. (1997) Structural organization and
regulation of the nif genes of Herbaspirillum seropedicae. Soil. Biol. Biochem., 29: 843-846.
Book
Chapters
Ishida, M.L., Machado, H.B., Souza, E.M., Yates, M.G., Pedrosa, F.O. Regulation of
nitrogen fixation in Azospirillum
brasilense involves the two-component regulatory proteins NtrY and
NtrX in nitrogen fixation: From Molecules to Crop Productivity Ed: Kluwer
Academic Press, 2000.
Ishida,
M.L.
2003. Molecular biology and plant-host interaction.
In: Leite, B., Pacholati, S.F. & Stangarlin, J. R.
Plant-Microbe Interactions: Physiology, Biochemistry and Molecular
Biology. University of São Paulo. (in press). (portuguese).
Non-referred
Publications
Alves, E. ,
Pascholati, S.F. Ishida, M. L. & Leite,
B. 2001. Bacteria: Xylella
fastidiosa. Leo Microscopy Magazine Leo Microscopy Magazine.
Abstracts
Leite, B., Ishida, M.L, Brodbeck, B., Marques, L., Olson M. E, Braga, M. R. & Andersen, P.C. 2004. Calcium bridging is critical for Xylella fastidiosa aggregation. Phytopathology 94: S59.
Leite, B., Andersen, P.C. & Ishida,
M. . 2003. Nutritional effects on colony size and biofilm
formation of Xylella fastidiosa growing on simple
chemically-defined media. B. Phytopathology 93:S50.
Leite, B., Ishida, M.L., &
Andersen, P.C. 2003. Effect of hydrophobicity and divalent ions on
biofilm formation of Xylella fastidiosa. Phytopathology
93:S50.
Ishida,
M.L.,
Machado, H.B., Souza, E. M., Yates, M.G., Pedrosa, F.O. (1998)
Identification of a new nitrogen regulatory genes ntrYX in Azospirillum
brasilense, XXVII Reunião
Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular, Caxambú,
MG, Brazil.
Leite, B.,
Ishida, M.L., Alves. E., Carrer,
H., Kitajima, E.W. & Pascholati, S.F. (2001). Evidence on the
involvement of sulfur and calcium in the adhesion of Xylella fastidiosa
from citrus. Fitopatologia Brasileira, 26: 546.
Ishida,
M.L.,
Souza, E. M., Machado, H. B., Yates, M. G., Pedrosa, F. O. (1997). Control of the activity of the NifA
protein in Azospirillum brasilense and Klebsiella pneumoniae.
XXVI
Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia
Molecular, Caxambú, MG, Brazil.
Ishida,
M. L.,
Souza, E. M., Rigo, L. U., Funayama, S. & Pedrosa, F. O. (1996). The
NifA protein from Azospirillum brasilense activates the nifH
promoters from Azospirillum brasilense,
XXV Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia
Molecular, Caxambú, MG, Brazil.
Ishida,
M L.,
Souza, E. M., Rigo, L. U., Funayama, S. & Pedrosa, F. O. (1995).
Functional analysis of the nifA of Azospirillum brasilense,
XXIV Reunião Anual da Sociedade Brasileira de Bioquímica, Caxambú, MG,
Brazil.
Ishida,
M L.,
Souza, E. M., Rigo, L. U., Funayama, S. & Pedrosa, F. O. (1995). Functional analysis NifA
protein from Azospirillum brasilense. International Symposium on
Sustainable Agriculture for the Tropics: The role of Biological Nitrogen
Fixation, Angra dos Reis, RJ, Brazil.
Fanta,
E., Grötzner, S. R., Luvizotto, M. F., Ishida, M. L.,
Wachovicz, L. M. (1991). The
environmental impact and the behavior of antarctic fish: I - Lighit,. Scar
International Conference on “Antarctic Science”- “Global
Concerns”, Bremen, Germany.
Fanta, E., Luvizotto, M. F., Grötzner, S. R., Meyer A.
A., Ishida, M. L., Wachovicz, L. M. (1991). The
environmental impact and the behavior of antarctic fish: II - Salinity,
setembro/1991. Scar International Conference on “Antarctic Science”-
“Global Concerns”, Bremen, Germany.
Fanta,
E., Ishida, M. L., Wachovicz, L. M., Luvizotto, M. F., Grötzner,
S. R. (1990). Preliminary studies of behavior and circadian rhythms of
Antarctic’s fishes, 42° Reunião Anual da Sociedade Brasileira para o
Progresso da Ciência,Natal , RN, Brazil (portuguese).
Ishida,
M. L.,
Luvizotto, M. F., Grötzner, S. R., Wachovicz, L. M.,
Fanta, E. (1990). Environmental behavior of Trematomus newnesi
and Notothenia gibberifrons during the end of Summer/1990, II Ciclo
de Atualização em Ciências Biológicas e III Mostra de Pesquisa na
Graduação de Biologia, Curitiba, PR, Brazil (portuguese).
Luvizotto,
M. F., Ishida, M. L., Grötzner, S. R., Wachovicz, L. M.,
Fanta, E. (1990). Biological rhythms of Antarctic’s fishes: mobility,
aggressively and inter and intra specifics contact, II Ciclo de Atualização
em Ciências Biológicas e III Mostra de Pesquisa na Graduação de
Biologia, Curitiba, PR, Brazil (portuguese).
Wachovicz,
L. M., Grötzner, S. R., Luvizotto, M. F., Ishida, M. L.,
Fanta, E. (1990). Comparison
between circadian rhythms profiles of Trematomus newnesi and Notothenia
gibberifrons. IX Encontro Brasileiro de Ictiologia, Maringá, PR, Brazil (portuguese).
Luvizotto,
M. F., Ishida, M. L., Grötzner, S. R., Wachovicz, L. M.,
Fanta, E. (1990). Influence of alteration of salinity in the antarctic
fish Notothenia gibberifrons, IX Encontro Brasileiro de Ictiologia,
Maringá, PR, Brazil (Portuguese).
Ishida,
M. L.,
Luvizotto, M. F., Grötzner, S. R., Wachovicz, L. M. (1990). Comparative
study of the gills of Antarctic’s fishes Notothenia gibberifrons
and Trematomus bernacchii, IX Encontro Brasileiro de Ictiologia,
Maringá, PR, Brazil (portuguese).
Fanta,
E., Grötzner, S. R., Ishida, M. L., Vasconcelos, V. L.,
Cavalcanti, M. C., Wachovicz, L. M., Luvizotto, M. F., Klaumann, R. J.
(1989). Effect of temperature and acid pH water in the respiratory system
of Trichogaster trichopterus (Pisces, Teleostei), 41° Reunião
Anual da Sociedade Brasileira para o Progresso da Ciência, Fortaleza, CE,
Brazil (portuguese).
Fanta,
E., Stofella, D. R. E., Mehler, B., Ortolani, C. F., Wachovicz, L. M., Grötzner,
S. R., Ishida, M. L. (1989). Sensorial structures from
modified pelvic fin of Trichogaster trichopterus (Pisces, Teleostei),
XII Colóquio da Sociedade Brasileira de Microscopia Eletrônica,
Caxambú, MG, Brazil (portuguese).
Grötzner, S. R., Ishida, M. L.
& Woehl, O. M. (1988) Morpho-functional study of modified pelvic fin
from Trichogaster trichopterus (Pisces, Teleostei). 1° Mostra de Pesquisa na Graduação do Curso de
Biologia, Curitiba, PR, Brazil (portuguese).
Vasconcelos,
V.L., Cavalcanti, M.C., Borges, R. J., Grötzner, S. R., Ishida, M.L.
& Woehl, O. M. (1988). Circadian Rhythm behavior of Trichogaster trichopterus (Pisces,
Teleostei). 1°
Mostra de Pesquisa na Graduação do Curso de Biologia, Curitiba, PR,
Brazil (portuguese).
Cavalcanti,
M. C., Ishida, M. L., Grötzner, S. R., Sene, A. L. A. De,
Uceda, R. A., Borges, R. J.,
Vasconcelos, V. L., Galli, R. M., Wachovicz, L.M., Ono, J. A., Santa
Cruz, E. J. M. & Mehler, B. (1988). Respiratory behavior study of
Trichogaster trichopterus (Pisces, Teleostei). 1°
Mostra de Pesquisa na Graduação do Curso de Biologia, Curitiba, PR,
Brazil (portuguese).
Fanta,
E., Grötzner, S. R., Ishida, M. L., Borges, R. J.,
Vasconcelos, V. L., Cavalcanti, M. C. & Woehl, O. M. (1988) Swimming
movements study in the behavior of Trichogaster trichopterus
(Pisces, Teleostei), 40° Reunião Anual da Sociedade Brasileira para o
Progresso da Ciência, São Paulo, SP, Brazil (portuguese).
Fanta, E., Vasconcelos, V. L., Santa Cruz, R. J., Ishida, M.L., Grötzner, S. R., Cavalcanti, M. C., Mehler, B., Wachowicz, L. M., Borges, R. J. (1988) Respiratory behavior of Trichogaster trichopterus (Pisces, Teleostei), VI Encontro Anual de Etologia, Florianópolis, SC, Brazil (portuguese).
Scientific expedition
VIII Brazilian Expedition to Antarctica (King George Island base). Jan/Feb, 1990
Graduate
Student Representative
Graduate
Student Representative in the Biochemistry and Molecular Biology
Department (Federal University of Paraná). (1997-1998).
Graduate
Student Representative in the Biochemistry graduate course in the
Biochemistry and Molecular Biology Department (Federal University of Paraná).
(1994-1995).