Breno Leite
Microanalysis Applications Scientist.
Email: Breno.Leite@thermofisher.com (for EDS only)
Additional Email accounts: bleite@cbbase.info ; bleitehome@hotmail.com
Associate Scientist - Plant Pathology - University of Florida. 2005-2007
North Florida Research and Education Center
Research Associate. 2001-2005
University of Florida – Quincy – FL
North Florida Research and Education Center
Institute of Food and Agricultural Sciences
Pathogenicity of Xylella
fastidiosa on grapes
Associate
Professor (tenured). Federal University of Paraná – UFPR. 1992-1998.
Department
of Biochemistry – Curitiba, PR, Brazil.
Coordinator
of the Biochemistry and Physiology of Plant Disease Laboratory.
Laboratory
Technician - "Adolfo
Lutz" Institute, Campinas, SP, Brazil, 1979.
"Trainee" Johnson & Johnson – Sumaré, SP, Brazil, 1978.
Pos-doctoral
Research
- 1998 - 2000
University of São Paulo (USP), Piracicaba, SP, Brazil.
Department of Entomology, Plant Pathology and
Agricultural Zoology
Electron Microscopy Center (NAP/MEPA)
Pathogenicity of Xylella fastidiosa: Functional genomics program
Education
PhD in Plant Pathology
- 1988 - 1992
Purdue University - Botany & Plant Pathology
Department.
MSc in Genetics
of
Microorganisms
-
1983 - 1987
State University of Campinas
(UNICAMP), Campinas, SP, Brazil.
BS Biology
- 1980 - 1983
State University of Campinas
(UNICAMP), Campinas, SP, Brazil.
Special training
1 - Project: "Use of Phage Display technology in search for surface features implicated in the germination of Fusarium verticilioides conidia". University of Missouri - Columbia, MO - Dept. of Plant Pathology and Microbiology. 2006. US.
2 - Project: "Development of a minimal defined medium for Xylella fastidiosa, the causal agent of Pierce's disease of grapevines". Training period at the University of Georgia. Experiment Station. Department of Plant Pathology, Griffin, GA. 2001. US.
3 - Project: "Scanning Electron Microscopy and X ray microanalysis of Citrus plants infected with Xylella fastidiosa, the causal agent of CVC (Citrus Variegated Chlorosis)". Microscopy Center, ESALQ- University of Sao Paulo. 1999. Brazil.
General
Interests
1 - Chemistry and biochemistry of cotton bolls exhibiting hardlock. Molecular identification of Fusarium verticilioides. Current.
2 - Use of Phage-display technology to uncover surface properties associated with germ tube formation of Fusarium verticilioides. Current.
3 - Germ tube and appressorium formation of uredospores of Phakopsora pachyrhizi, causal agent of soybean rust. Current.
4
- Adhesion mechanisms of the pathogenic bacteria and fungi. Xylem chemistry and its
influence in pathogenicity.
5 - Characterization of plant pathogenic fungi through scanning and
transmission electron microscopy, X ray microanalysis and fluorescence
microscopy.
6 - Adhesion, germination and appressoria formation of plant pathogenic fungi.
7
- X ray microanalysis techniques applications on plant-pathogen
interactions.
Professional Associations
Brazilian Society of Biochemistry and Molecular Biology
- Brazil
American Phytopathological Society
– USA
The Science Advisory Board - USA Current
Brazilian Society of Microscopy and Microanalysis
– Brazil
American Society of Microbiology - USA Current
Brazilian Society of Genetics - Brazil
Brazilian Society of Cell Biology - Brazil
Publications
Publications
in Peer Reviewed Journals
Marois, J. J., Wright, D. L, Leite, B., Mailhot, D. & Osekre, E. 2007. Hardlock of Cotton: Historical Review and Perspectives. WCR-4.
Andersen, PC., Brodbeck B. V., Oden S., Shriner. A., Leite, B. 2007. Influence of xylem fluid chemistry on planktonic growth, biofilm formation and aggregation of Xylella fastidiosa. FEMS Microbiology Letters Vol. 274: 210.
Ishida, M.L.., Andersen, P.C.& Leite, B. 2004. Cecropin B antimicrobial activity against Xylella fastidiosa: effect on colony formation. Physiological and Molecular Plant Pathology. 64:73-81.
Leite, B.,
Andersen, P.C. & Ishida, M.L. 2004. Colony aggregation and biofilm
formation in xylem chemistry-based media for Xylella fastidiosa.
FEMS Microbiol. Lett. 230:283-290.
Alves, E., Marucci, R.C., Lopes, J.R.S. & Leite, B., 2004. Relationship between the proportion of colonized vessels by Xylella fastidiosa in plum, coffee and citrus and the leaf symptomatology exhibited. J. Phytopathology. 152: 291-297.
Andersen, P.C., Momol, E.A., Leite, B., Momol, M.T Ishida, M.L. 2004. The Effect of Lytic Peptides on Xylella fastidiosa in buffer and in Xylem Fluid Grapevines. Vitis 43: 19-25.
Alves,
E., Kitajima, E.W. & Leite, B.
2003. Interaction of Xylella
fastidiosa with different cultivars of Nicotiana
tabacum: a comparison of colonization patterns". J.
Phytopathology. 151:500-506.
Schadeck, R.J.G., Randi,
M.A.F., Buchi, D.F. & Leite, B. 2002. Vacuolar
system of ungerminated Colletotrichum
graminicola conidia: Convergence of autophagic and endocytic pathways.
FEMS Microbiol. Lett. 218:
277-283.
Leite, B., Ishida, M. L., Alves, E.1, Carrer, H., Pascholati, S. F. & Elliot W. Kitajima. 2002. Genomics and X-ray Microanalysis Indicate that Ca++ and thiol mediate the Xylella fastidiosa Aggregation and Adhesion. Braz. J. of Med. and Biolog. Sci. 36:645-650.
Leite,
B.
Pascholati, S.F., Kitajima, E. W. & Ishida, M. L. 2001. Mechanisms
of adhesion of bacteria and fungi to host plants. Annual Review of
Plant Pathology. 9:
119-157 (portuguese).
Schadeck, R.J., Buchi, D. & Leite, B. 2000. Acid phosphatase activity in ungerminated conidia of Colletotrichum graminicola as determined by spectrophotometric and cytochemical methods. Braz. J. Morphol. Sci. 17: 11-15.
Yara, R., Azevedo, J. L., Macheroni, Jr. W., Horii, J.,
Kitajima, E. W. & Leite, B. 1999. Mycoplasma
associated with cultures of the ligno-celulolitic fungus Pleurotus sp.
Acta Microscopica.
8:851-852.
Schadeck,
R. J. G., Buchi, D. F & Leite,
B. 1998.
Ultrastructural
aspects of Colletotrichum graminicola conidium formation and
penetration on cellophane membranes: focus on lipid reserves. J.
Submicrosc. Cytol. Pathol. 30: 555-561.
Schadeck, R. J. G., Leite, B. &
Buchi, D. F. 1998. Lipid
mobilization and acid phosphatase activity in lytic compartments during
conidium dormancy and aprressorium formation of Colletotrichum
graminicola. Cell Structure
and Function. 23: 333-340.
Sugui, J. A., Leite,
B. &
Nicholson, R. L. 1998. Partial characterization of the extracellular
matrix released onto hydrophobic surfaces by conidia and conidial
germlings of Colletotrichum
graminicola. Physiological and
Molecular Plant Pathology 52:
411-425.
Pascholati, S.F. Stangarlin, J. R. Leite,
B. & Schwan-Estrada, K. R. F. 1998. Mechanisms of pathogenicity in
fungi. Annual Review of Plant pathology. 6: 1-47. (portuguese).
Leite, B.,
Roncato, L.D.B., Pascholati, S.F. & Lambais, M. 1997. Recognition
and molecular signal transduction in plant-pathogenic fungus interactions.
Annual Review of Plant pathology. 5: 235-280. (portuguese).
Oliveira, R.F.; Pascholati, S.F. & Leite,
B. 1997. Papilla formation and peroxidase activity in Mimosa
scabrella hypocotyls inoculated with the non-pathogen Colletotrichum graminicola. Fitopatologia
Brasileira 22:
195-197.
Schadeck,
R.J.G., Leite, B.
& Buchi, D.F. 1996. Morphological,
biochemical and cytochemical studies during germination of Colletotrichum graminicola conidia. Brazilian Journal of Morphological Sciences. 5 (A): XI – XLV.
Leite, B. &
Schadeck, R.J.G. 1996. Mycosporines: their importance in the process of
fungal spores germination. Fitopatologia Brasileira.
21: 149-155.
Leite, B.,
Wulff, N.A., Alquini, Y., Pascholati, S.F. & Oliveira, R.F. 1995.
Passiflora edulis X Colletotrichum gloeosporioides
and Mimosa scabrella X Colletotrichum
graminicola: a comparative study of a compatible and a non-compatible
interaction. Summa
Phytopathologica. 21:
87-90.
Leite, B.,
Sander, P. & Sugui, J. A. 1995. O
papel de compostos voláteis na germinação
de esporos fúngicos e na expressão de doenças em plantas.
Summa Phytopathologica.
21: 109-116.
Pascholati, S.F. & Leite, B. 1994. Biochemical mechanisms of resistance to
diseases. Annual Review of Plant pathology. 2:
1-51 (portuguese).
Mercure,
E.W., Leite, B. &
Nicholson, R.L. 1994. Adhesion of ungerminated conidia of Colletotrichum
graminicola to artificial hydrophobic surfaces. Physiological
and Molecular Plant Pathology.
45: 421-440.
Pascholati, S.F., Deising, H., Leite, B., Anderson, D. & Nicholson, R.L. 1993. Cutinase
and non-specific esterase activities in the conidial mucilage of Colletotrichum
graminicola. Physiological
and Molecular Plant Pathology.
42: 37-51.
Leite, B.
1993. Controle de germinação em Colletotrichum
graminicola: mecanismos e perspectivas.
Summa
Phytopathologica.
19: 55-59.
Leite, B.
& Nicholson, R.L. 1993. Production of a volatile self-inhibitor by Colletotrichum
graminicola.
Mycologia
85: 945- 951.
Ransom, R.F., Hipskind, J.D., Leite, B. Nicholson, R.L. & Dunkle, L.D. 1992. Effects of elicitor from Colletotrichum graminicola on
the response of sorghum to Periconia
circinata and its
pathotoxin.
Physiological
and Molecular Plant Pathology.
41: 75-84.
Hipskind, J.D., Wood, K.V., Leite, B. Chand, T., Lembi, C.A. & Nicholson, R.L. 1992.
A fungitoxic phenolic compound in Hydrilla
verticilata. Biological
Control. 2: 51-58.
Leite, B.
& Nicholson, R.L. 1992. Mycosporine-alanine, a self-inhibitor of
germination from the conidial mucilage of Colletotrichum
graminicola.
Experimental Mycology. 16: 76-86.
Snyder, B.A., Leite,
B. Hipskind, J.D., Butler, L.G. & Nicholson, R.L. 1991.
Accumulation of sorghum phytoalexins induced by Colletotrichum graminicola at the infection site. Physiological
and Molecular Plant Pathology. 39: 463- 470.
Hipskind, J.D., Hanau, R. Leite, B. & Nicholson, R.L. 1990. Phytoalexin
accumulation in sorghum: identification of an apigeninidin acyl ester. Physiological
and Molecular Plant Pathology. 36: 381-396.
Conference Proceedings
Andersen,
P. C., Leite,
B. & Ishida, M. L. 2005. Xylem
chemistry mediation of resistance to Pierce`s disease. Pierce`sDisease
Control Program.
E. Alves & Leite, B. 2004. Aspects of Xylella fastidiosa xylem vessel colonization of sweet orange (Citrus sinensis (L. Osbeck) cultivar “Pêra”, revealed by electron microscopy. Microsc Microanal 10 (Suppl 2):1452-53
Andersen, P. C., Leite, B. & Ishida, M. L. 2004. Xylem chemistry mediation of resistance to Pierce`s disease. Pierce`s Disease Control Program. San Diego, CA, USA. Symposium Proceedings. Pages 3-6.
Andersen, P. C., Leite, B. & Ishida, M. L. 2003. Xylem chemistry mediation of resistance to Pierce`s disease. Pierce`s Disease Control Program. San Diego, CA, USA. Symposium Proceedings. Pages 3-5.
Leite,
B. 2001.
Adhesion
and aggregation of plant pathogenic bacteria: importance in pathogenicity. Annals of the São Paulo Plant Pathologists group.
XXVI congress. (portuguese).
Leite,
B. Ishida, M.L.,
Alves, E., Pascholati, S.F. & Sugui, J. A. 2000. Detection of calcium in the adhesive material obtained from the plant
pathogen Colletotrichum graminicola: X ray microanalysis (EDS)
evidences. Proceedings of Microscopy and Microanalysis. 6: 698-9.
Honors / Distinctions
1.
Published the “image
of the week” at the
2. Sigma XI, The Scientific Research Society: Granted Full membership (2006). http://www.sigmaxi.org/.
3. Member of the National Education Association (Faculty of Florida Chapter).
4.
Student under my
supervision was classified among the 4 professional category distinguished posters (Lis R. Uliana)
during the XXIV Annual meeting of the São Paulo
State Plant Pathologists. 2001.
5.
Cover
of the Brazilian Journal of Morphological Sciences.
(Schadeck et al,17: 11-15. 2000).
6.
Cover of the
Microscopy & Analysis
magazine (Leite, B. & E.
W. Kitajima, Vol. 41, March, 2000).
7.
Student under my
supervision was awarded (First prize: agriculture
category) in a scientific initiation meeting (Douglas Lau), EVINCI –
Federal University of Paraná, Curitiba, PR, Brazil. 1996.
8.
Student under my supervision received a distinguished mention for his
work presented at a scientific initiation meeting. (Nelson A. Wulff),
EVINCI – Federal University of Paraná, Curitiba, PR, Brazil. 1994.
9. Publication was included in the 1993 Plant Disease highlights list (March, 1994 pag. 219): “Cultures of Colletotrichum graminicola produce a volatile self-inhibitory material that inhibits spore germination and prevents disease development in inoculated sorghum seedlings (Mycologia 85:945-951, 1993).
10.
Young Scientist Award granted to Breno Leite by the Brazilian Government (CNPq/Roberto
Marinho Foundation). Biological Control category, 1983.
Students
Eduardo
Alves – PhD, 2003.
Ruth Janice Guse Schadeck - PhD, 1999.
Janyce Akemi Sugui - PhD, 1998.
Lauren
Dal Bó Roncato - MSc, 1997
Rosimery
de Fátima Oliveira - MSc. 1994.
Translations
Kemmitt
Schumann
Wright,
S. (Leite, B.
translator) 1997. Phytotoxins as antimicrobial agents. Annual Review of Plant Pathology. 5: 157-197. (portuguese).
Book Reviewer
Miguel U. & Hanlin, R. T. 2000. Illustrated Dictionary of Mycology. American Phytopathological Society. 448 pages.
Alves, E. , Pascholati, S.F. Ishida, M. L. & Leite,
B. 2001. Bacteria:
Xylella fastidiosa. Leo Microscopy Magazine Leo Microscopy Magazine.
Leite,
B.
& Kitajima, E. W. 1999. Plant pathogenic fungi. Leo Microscopy Magazine.
White papers
Breno Leite, PhD & Stephen Leong, PhD. Pierce's Disease, a Problem in Florida Vineyards. October 02, 2004.
Breno Leite, PhD, Raymond Hix, PhD & Stephen Leong, PhD. F AMU' s Campus Wide Imaging Core. October 02, 2004.
Breno Leite, PhD & Stephen Leong, PhD. FAMU's Plant Disease Diagnosis Laboratory (PDDL). October 02, 2004
Technical Manuals
Leite,
B. &
Kitajima, E. W.1999. Scanning
Electron Microscopy and X ray microanalysis. Manual-NAP/MEPA.
38 p. (portuguese).
Kitajima,
E. W. & Leite, B. 1999. Scanning
Electron Microscopy. 2
nd Edition. Manual-NAP/MEPA. 46 p. (portuguese)
Kitajima,
E. W. & Leite, B. 1999. Variable
pressure Scanning Electron Microscopy. Manual-NAP/MEPA.
43 p. (portuguese)
Book Chapters
Pascholati, S. & Leite, B. 1995. "Hospedeiro: Mecanismos de Resistência". In: Manual
de Fitopatologia, ESALQ-USP. Ed. Ceres. Pags. 417-452.
Leite, B.
& Pascholati, S. F. 1995. Hospedeiro: "Alterações fisiológicas
nas interações com patógenos" In: Manual de Fitopatologia,
ESALQ-USP. Ed. Ceres. Pags. 393-414.
Leite, B. & Stangarlin, J. R.
Fungal
pathogenicity towards plants. IN: Physiology, Biochemistry and
Molecular Biology of Plant-pathogen interactions (Leite,
Pascholati & Stangarlin eds). (in press). (portuguese).
Alves, E. Leite, B.
& Kitajima, E. W. Ultrastructure
in era of DNA. IN: Physiology, Biochemistry and
Molecular Biology of Plant-pathogen interactions (Leite,
Pascholati & Stangarlin eds). (in press). (portuguese).
Stangarlin, J. R & Leite, B
Host
alterations during pathogenicity. IN: Physiology, Biochemistry and
Molecular Biology of Plant-pathogen interactions (Leite,
Pascholati & Stangarlin eds). (in press). (portuguese).
Abstracts
Leite, B., Marois, J. J., Wright, D. L., Mailhot, D. J. & . Osekre, E. 2007. Etiology and Management of Fusarium hardlock of cotton in Florida. Phytopathology,97: S63.
Leite, B., Marois, J. J., Wright, D. L., Mailhot, D. J. & E. Alves. 2007. Hardlock symptom types, fiber quality and molecular identification of Fusarium isolates. Phytopathology,97: S63.
Leite, B., Marois, J. J., Wright, D. L., Mailhot, D. J. & . Osekre, E. 2007. Naturally infected and inoculated cotton flowers: A Fusarium hardlock investigation. Phytopathology,97: S63.
Leite, B., Mailhot, D. J. Marois, J. J., Wright, D. L. 2007. Growth, conidial germination and fumonisin production of Fusarium verticillioides on cottonseed agar, cotton flower agar and potato dextrose agar. Phytopathology,97: S63.
Leite, B., Narvaez, D., J. Marois, J. J., Wright, D. L. 2007. Clumping of Phakopsora pachyrhizi urediniospores and its significance to spore biology. Phytopathology,97: S63.
Leite, B., Marois, J. J., Wright, D. L., & Mailhot, D. J. 2007. Development of Fusarium in Cotton Bolls: Greenhouse and Field Results" - Proceedings of the Belwide Cotton Conferences, New Orleans, Louisiana, January 9-12, 2007.
Leite, B., Marois, J. J., Wright, D. L., & Mailhot, D. J. 2007. Relationship Between Flower Visitors and Cotton Hardlock Greenhouse and Field Results" - Proceedings of the Belwide Cotton Conferences, New Orleans, Louisiana, January 9-12, 2007
Leite,
B., P. C.
Andersen. & E. W. Kitajima. 2005. Induction of “in
vitro” aggregation of
Xylella fastidiosa cells
by divalent ions. Proceedings of thePierce`s disease symposium.
Leite,
B., L. Marques, P. C. Andersen & M. Olson. Xylella
fastidiosa growth
on chemically-defined media: CHARD2, 3G10R and XF-26. Proceedings of the
Pierce`s disease symposium.
Leite,
B., P. C.
Andersen. 2005. The influence of cell suspension redox potential on the
capacity of Xylella fastidiosa to aggregate.
Proceedings of the Pierce`s disease symposium.
Leite,
B., P. C.
Andersen & M. L. Ishida. 2005. Comparative study of Xylella
fastidiosa surface proteins exhibiting high contents of cysteine
residues: significance in pathogenicity. Proceedings of the Pierce`s
disease symposium.
Leite,
B. and
Andersen, P. C. 2005. Effects of reduced and oxidized glutathione on
calcium bridging during the aggregation of Xylella
fastidiosa.Phytopathology 95:963.
Leite,
B, Andersen,
P. C. and M. L. Ishida.2005.
In silico analysis of Xylella
fastidiosa searching for surface proteins bearing high contents of
cysteines (thiol) residues: importance in pathogenicity. Phytopathology
95:962.
Leite,
B, Andersen,
P. C. and Kitajima, E. W. 2005. Calcium and magnesium influence on
the aggregation of Xylella
fastidiosa, assessed by
image analysis of SEM micrographs. Phytopathology 95:963.
Marques,
L., Leite, B.,
Andersen, P. C. and Olson, M. 2005.Plaktonic and biofilm growth
of Xylella fastidiosa
in chemically-defined media. Phytopathology 95:963.
Leite, B., Ishida, M. L., Brodbeck, B., Marques, L., Olson M. E, Braga, M. R. & Andersen, P.C. 2004. Calcium bridging is critical for Xylella fastidiosa aggregation. Phytopathology 94: S59.
Leite,
B., P.C. Andersen, and M.L. Ishida. 2003. Nutritional
effects on colony size and biofilm formation of Xylella fastidiosa
growing on simple chemically-defined media. B. Phytopathology 93:S50.
Leite,
B., M.L. Ishida, and P.C. Andersen. 2003. Effect of
hydrophobicity and divalent ions on biofilm formation of Xylella
fastidiosa. Phytopathology 93:S50.
E.
Alves, S.F. Pascholati 2 , E.W. Kitajima & Leite,
B. 2003. Interaction
of Xylella fastidiosa with different varieties of Nicotiana
tabacum: A comparison of colonization patterns. Phytopathology 93:
S50.
Alves, E., R.C. Marucci , S.F. Pascholati , J.R.S. Lopes, Leite, B. 2003. Relationship between leaf symptoms and the proportions of xylem vessels of plum, coffee and citrus colonized by Xylella fastidiosa. Phytopathology 93: S50.
Alves.
E., Pascholati, S.F. & Leite,
B. 2001. Varieties of Nicotiana
tabacum as alternative experimental hosts for studies of the
interaction plant - Xylella fastidiosa. Fitopatol. Bras. 26: 271. (Portuguese).
Alves.
E., Wulff, N.A., Pascholati, S.F. & Leite,
B. 2001 Xylella fastidiosa preferentially colonizes pitted
vessels: significance in pathogenicity. Fitopatol. Bras. 26: 271.
Alves.
E., Pascholati, S.F. & Leite,
B. 2001. Formation of bacterial biofilms on the surface of
polystyrene: a methodology for the scanning electron microscope. Acta
Microscopica. Annals of the Microscopy Meeting. 789.
Wulff,
N.A., Alves. E., Leite, B.,
& Pascholati, S.F. 2001. Cellulases of Xylella fastidiosa:
expression, catalytic activity and colonization of xylem cells. Fitopatol.
Bras., 26:287. (Portuguese).
Leite,
B.,
Ishida, M.L., Alves. E., Carrer, H., Kitajima, E.W. & Pascholati, S.F.
2001. Evidence on the involvement of sulfur and calcium in the adhesion of
Xylella fastidiosa from citrus. Fitopatol.
Bras. 26: 546.
Yara, R.,
Azevedo, J.L., Maccheroni Jr., W., Hori, J., Kitajima, E.W. & Leite,
B. Mycoplasma associated
with cultures of the ligno-cellulolytic Fungus Pleurotus sp.
Proc.XVII Cong. Soc. Bras. Microsc. Microanálise. X Cong. Soc. Bras.
Biol. Cel. (Santos, 10/99). Acta Microscopica 8: 851-852. 1999.
Yara,
R., Azevedo, J.L., Horii, J., Kitajima, E.W. & Leite, B. Detection of mycoplasmas associated to culture of
the ligno-cellulolitic fungus Pleurotus sp. Res. XX Cong. Bras.
Microbiologia (Salvador, BA, Out/99). MB-187. P. 219. 1999. (Portuguese).
Yara,
R., Azevedo, J.L. & Leite,
B. Optical microscopy and electron microscopy of the interaction Pleurotus
sp. and sugar cane bagass. Res. XX Cong. Bras. Microbiologia (Salvador,
BA, Out/99). MB-167. P. 214. 1999. (Portuguese).
Carvalho
Jr., A., & Leite, B.
Confocal microscopy and scanning electron microscopy of Puccinia
recondita Rob. Ex Desm. in herbarized wheat plants (res.164).
Fitopatol. Bras. 24: 272. 1999. (Portuguese).
Leite,
B.
& Novaes, Q.S. Observation of rust (Hemileia vastatrix) in
coffee leaves: comparison of results obtained in conventional and high
pressure scanning electron microscopy. Fitopatol. Bras. 24: 297. 1999.
(Portuguese).
Leite,
B.
Schadeck, R.J.G. Di Piero, R.M. & Pascholati, S.F. Mycosporine-alanine,
a metabolite isolated from Colletotrchum graminicola, does not
induce the accumulation of phytoalexins in sorghum. Fitopatol. Bras. 24:
297. 1999. (Portuguese).
Leite,
B. &
Lau, D. 1997. Potential alternative for the evaluation of susceptibility
and/or resistance to soybean stem canker through the use of inoculated
cotyledons. XXX Congresso Brasileiro de Fitopatologia. Fitopatol. Bras.
22: 275. (Portuguese).
Lau,
D., Alquini, Y. & Leite, B.
1997. Penetration steps of Phomopsis phaseoli f. sp. meridionalis
in resistant soybean hypocotyls to the stem canker. XXX
Congresso Brasileiro de Fitopatologia. Fitopatol. Bras.
22: 274. (Portuguese).
Roncato,
L.D.B., Chubatsu, L.S., Delboni, L.F., Pedrosa, F.O. & Leite, B. 1997. Peroxidase activity and isoenzymatic patterns
in extracts from susceptible and resistant varieties to soybean stem
canker. XXX Congresso Brasileiro de Fitopatologia. Fitopatol. Bras. 22:
303. (Portuguese).
Roncato, L.D.B.,
Chubatsu, L.S., Delboni, L.F., Pedrosa, F.O. & Leite, B. 1997. Fourier Transform infrared monitoring of the Colletotrichum
graminicola adhesion. XXVI – Reunião anual da Sociedade Brasileira
de Bioquímica e Biologia Molecular. Caxambú – MG. Annals of the
meeting, pag. 40.
Lau,
D., Roncato, L.D.B., Terasawa, L.G. & Leite,
B. 1996. Production of gliceoline and morphological aspects
in tissues of two varieties of soybean inoculated with Diaporthe
phaseolorum. XXIX
Congresso Nacional de Fitopatologia, Campo Grande - MS. Fitopatol. Bras.
21:400. (Portuguese).
Roncato,
L.D.B., Lau, D. & Leite, B.
1996.Evaluation of peroxidase activity in extracts obtained from
resistant and susceptible soybean varieties inoculated with the fungus Diaporthe
phaseolorum. XXIX Congresso Nacional de Fitopatologia, Campo Grande -
MS. Fitopatol. Bras.
21:406. (Portuguese).
Schadeck,
R.J.G., Buchi, D.F. & Leite,
B. 1996. Ultrastructural
aspects of Colletotrichum graminicola appressorium formation on
cellophane membranes XXIX
Congresso Nacional de Fitopatologia, Campo Grande- MS. Fitopatol. Bras. 21:407. (Portuguese).
Sugui, J.A., Leite,
B., Schadeck, R.J.G. & Janissek, P.R. 1996. Colletotrichum graminicola adhesion monitored by Furier transform infrared
spectroscopy (FTIR). XXIX Congresso Nacional de Fitopatologia, Campo
Grande- MS. Fitopatol.
Bras. 21:408. (Portuguese).
Schadeck,
R.J.G. & Leite, B.
1996. Morphological changes and phosphatase activity during conidial
germination of Colletotrichum graminicola. XXV Reunião Anual da
Sociedade Brasileira de Bioquímica e Biologia Molecular. Caxambu - MG.
(Annals of the meeting, pag. 35).
Sugui,
J.A.; Leite, B.;
Schadeck, R.J.G. & Janissek, P.R. 1996. Fourier transform infrared
monitoring of the Colletotrichum graminicola adhesion. XXV Reunião
Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular. Caxambu
- MG. (Annals of the meeting, pag. 54).
Schadeck,
R.J.G.; Buchi, D.F. & Leite,
B. 1996. Ultrastructural and morphological changes during conidial
germination of Colletotrichum graminicola. IX Brazilian Congress of
Cell Biology. Águas
de Lindóia , SP. Braz. J. morphol. Sci.
13: 154.
Schadeck,
R.J.G., Buchi, D.F. & Leite,
B. 1996. Ultrastructural and morphological changes during conidial
germination of Colletotrichum graminicola. IX Brazilian Congress
of Cell Biology. Águas
de Lindóia – SP. Braz. J. morphol. Sci. 13: 154.
Sugui,
J.A., Leite, B. Schadeck,
R.J.G. & Janissek, P.R. 1996. Fourier Transform infrared monitoring of
the Colletotrichum graminicola adhesion. XXV – Reunião anual da
Sociedade Brasileira de Bioquímica e Biologia Molecular. Caxambú – MG.
(Annals of the meeting, pag. 54).
Schadeck,
R.J.G. & Leite, B.
1996. Morphological changes and phosphatase activity during
conidial germination of Colletotrichum graminicola. XXV – Reunião
anual da Sociedade Brasileira de Bioquímica e Biologia Molecular. Caxambú
– MG.
Sugui, J.A., Leite,
B. Schadeck, R.J.G. & Janissek, P.R. 1996. Colletotrichum graminicola adhesion monitored by Furier transform infrared
spectroscopy . XXIX – Congresso Nacional de Fitopatologia – Campo
Grande – MS. Fitopatol.
Bras. 21: 408. (Portuguese).
Schadeck,
R.J.G., Buchi, D. & Leite,
B. 1996. Ultrastructural aspects of Colletotrichum graminicola
of the appressoria formation on cellophane membranes. XXIX – Congresso
Nacional de Fitopatologia – Campo Grande – MS. Fitopatol. Bras. 21:
407. (Portuguese).
Roncato,
L.D.B., Lau D., & Leite, B.
1996. Evaluation of peroxidase activity in extracts obtained from soybean
resistant and susceptible varieties inoculated with the fungus Diaporthe
phaseolorum. XXIX – Congresso Nacional de Fitopatologia – Campo
Grande – MS. Fitopatol. Bras. 21: 406. (Portuguese).
Lau,
D. Roncato, L.D.B., Terasawa, L.G. & Leite,
B. 1996. Glyceolin production and morphological aspects in tissues
of two varieties of soybean inoculated with the plant pathogen Diaporthe
phaseolorum. XXIX – Congresso Nacional de Fitopatologia – Campo
Grande – MS. Fitopatol. Bras. 21: 400).
Schadeck,
R.J.G., Nicholson, R.L. &
Leite, B. 1995.
Senescence, self-inhibition, lipid reserves and acid phosphatase activity
in conidia of Colletotrichum graminicola. XXVIII – Congresso
Nacional de Fitopatologia – Ilhéus – BA. Fitopatol. Bras.
20; 326. (Portuguese).
Wulff,
N.A., Alquini, Y. & Leite,
B. 1995. Histology and scanning electron microscopy in hypocotyl
leaves of Passiflora edulis inoculated with Colletotrichum
gloeosporiodes. XVIII Congresso Paulista de Fitopatologia. Piracicaba
– SP. Annals of the meeting. (Portuguese).
Oliveira,
R.F. & Leite, B.
1995. Preformed and induced defenses of
Mimosa scabrella against fungi. XVII Congresso Paulista de
Fitopatologia. Piracicaba – SP. Annals of the meeting. (Portuguese).
Wulff,
N.A. & Leite, B.
1995. Spectrophotometric analysis of different species of Passiflora
challenged by the pathogen Colletotrichum
gloesporioides and peroxidase activity in
Passiflora edulis flavicarpa. XVIII congresso Paulista de
Fitopatologia. Piracicaba – SP. (Portuguese).
Wulff,
N.A., Alquini, Y. & Leite,
B. 1995. Histology and scanning electron microscopy in leaves and
hypocotyls of Passiflora edulis inoculated with Colletotrichum
gloeosporioides. XVIII Congresso Paulista de Fitopatologia, Piracicaba,
SP. (Annals of the meeting, pag. 127). (Portuguese).
Schadeck,
R.J.G., Nicholson, R.L. & Leite,
B. 1995. Senescence, self-inhibition, lipid reserves and
phosphatase activity in conidia of the pathogen
Colletotrichum graminicola. XXVIII Congresso Nacional de
Fitopatologia, Ilhéus - SC. Fitopatol. Bras. 20: 326. (Portuguese).
Freitas Filho,
D. & Leite, B. 1994.
Comparative
study of mycosporine-alanine production of two isolates of Colletotrichum
graminicola. Annals of the meeting – UFPR. Curitiba – PR.
(Portuguese).
Wulff,
N.A., Alquini, Y. & Leite,
B. 1994. Biochemical and histological
changes observed in seedlings of Passiflora edulis inoculated with Colletotrichum
gloesporioides (pathogen) e Colletotrichum graminicola (non
pathogen). Anais do Evento de Iniciação Científica – UFPR. Curitiba
– PR. (Portuguese).
Mercure.
E., Leite, B. &
Nicholson. 1994. Adherence of non-germinated conidia of Colletotrichum
graminicola. XXVII congresso Nacional de Fitopatologia, Itajaí - SC. Fitopatol.
Bras. 19: 335. (Portuguese).
Oliveira,
R.F., Pascholati, S.F. & Leite,
B. 1994. Papilla formation, lignification and activity of
peroxidases in etiolated seedlings of Mimosa scabrella inoculated
with the non-pathogenic fungus Colletotrichum graminicola. XXVII
congresso Nacional de Fitopatologia, Itajaí - SC. Fitopatol. Bras. 19:
326. (Portuguese).
Pascholati,
S.F., Deising, H., Leite, B.,
Anderson. D. & Nicholson, R.L. 1993. Presence of non-specific
esterases in the conidial mucilage Colletotrichum. graminicola. XVI
Congresso Paulista de Fitopatologia, Campinas – SP. Summa
Phytopathologica 19: 31. (Portuguese).
Oliveira,
R., Baggio, A.J. Pascholati, S.F. & Leite,
B. 1993. Evidence of phenolic compounds with anti fungal activity
in leaf extracts Mimosa scabrella. XXVI Congresso Nacional de
Fitopatologia, Aracajú - SE. Fitopatol. Bras. 18: 269. (Portuguese).
Leite,
B.,
Mercure, E. & Nicholson, R.L. 1993. Adherence of Colletotrichum
graminicola conidia to sorghum leaves and surfaces covered with
polystyrene. XXVI
congresso Nacional de Fitopatologia, Aracajú - SE. Fitopatol.
Bras. 18: 284. (Portuguese).
Pascholati,
S.F., Deising, H. Leite, B.,
Anderson, D. & Nicholson, R.L. 1993. Cutinolitic esterasic activity in
the mucilagem of Colletotrichum graminicola, causal agent of corn
anthracnose. XXVI Congresso Nacional de Fitopatologia, Aracajú - SE.
Fitopatol. Bras. 18: 350. (Portuguese).
Leite, B.
Nicholson, R. L. 1993. Supression of disease development in sorghum
mesocotyls by a volatile inhibitor produced by Colletotrichum
graminicola. Reunião da American Phytopathological Society, Oregon,
EUA. Inoculum 43:40, 1993.
Reviewer
for Academic Journals
FEMS Microbiology
Fitopatologia Brasileira
Microbiology
Revisao
Anual de Patologia de Plantas
Revista
Brasileira de Botânica
Physiological and Molecular Plant Pathology
Scientia
Agrícola
Summa Phytopathologica
Reviewer
for Grant Proposals of Funding Agencies
United States Department of Agriculture
Conselho
Nacional de Desenvolvimento Científico e Tecnológico
Fundação
de Amparo à Pesquisa do Estado de São Paulo
Instituto Agronômico do Paraná
Reviewer
for Books and Book Chapters
APS press
Peer-Reviewer
for In-house Manuscripts
NFREC-IFAS
– University of Florida
Universidade
de São Paulo
Universidade Federal do Paraná
Selected Seminars
“Development
of Fusarium in Cotton Bolls: Greenhouse and Field Results” - Cotton
Conferences,
“Relationship
Between Flower Visitors and Cotton Hardlock Greenhouse and Field
Results” - Cotton Conferences,
“Aspects
of spore germination research”. Soybean Rust Identification Short
Course. North Florida Research and
“Xylella
fastidiosa: a pathogen of grapevines and a potential problem for the
citrus industry in
“Rhamnolipids,
biopesticides for 21st Century”. Seminar presented to
scientists from the
“Plant
Pathology at
“An
overview of Pierce`s disease and other common vineyard diseases in
Florida”. Grape Grower`s Field Day Program. Seminar presented to
Grape Growers visiting the CVSFR,
“Importance
of disease management in the vineyard” Springtime Considerations for
Muscadine Grape Production in
“
"Introduction to the new era in SEM". Seminar presented to the faculty of Florida A & M University at the Conference CenterMain Campus. Tallahasse - FL - USA. 11/09. 2004.
"Influence of xylem chemistry on Xylella fastidiosa pathogenicity". Seminar presented to the faculty and students of the Plant Pathology Department - University of Florida - Gainesville - FL - USA, 02/06, 2004.
"Adhesion and pathogenicity: from Colletotrichum graminicola to Xylella fastidiosa". Seminar presented to faculty and students of the Viticulture Center - Florida A & M University - Tallahasse - FL - USA. 12/12, 2003.
“Nutritional requirements of Xylella fastidiosa: implications in pathogenicity”. Seminar presented to the NFREC-University of Florida faculty. Quincy – FL – USA. 05/15, 2002.
“Adhesion and aggregation of plant pathogenic bacteria:
importance to pathogenicity”. Participant in a Symposium at the XXIV Congress of the São Paulo
Plant Pathologists Group. Piracicaba – SP- Brazil. 02/06-08,
2001.
“Biochemical
markers and resistance induction in plants”. Participant in an international Symposium
“Biochemical aspects of pathogenicity and resistance” during the
XXXI Brazilian Congress of Plant Pathology. Fortaleza, CE, Brazil.
08/09-14, 1998.
Scholarships